Composite

Part:BBa_K4121083

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-05)


Expression cassette of ERG12 with constitutive promoter

The part is called "pCCW12-ERG12-tPRM9". We use pCCW12 as the promoter, tPRM9 as the terminator and the CDS of this part is ERG12. The three basic parts above make up a new transcription unit, It has the function to catalyze the conversion of mevalonate to mevalonate-P in mevalonate pathway.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 240
    Illegal EcoRI site found at 1241
    Illegal XbaI site found at 1323
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 240
    Illegal EcoRI site found at 1241
    Illegal NheI site found at 1898
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 240
    Illegal EcoRI site found at 1241
    Illegal BglII site found at 1260
    Illegal BamHI site found at 804
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 240
    Illegal EcoRI site found at 1241
    Illegal XbaI site found at 1323
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 240
    Illegal EcoRI site found at 1241
    Illegal XbaI site found at 1323
    Illegal AgeI site found at 1555
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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