Composite
Part:BBa_K4121083
Designed by: Ziyu Li Group: iGEM22_NEFU_China (2022-10-05)
Expression cassette of ERG12 with constitutive promoter
The part is called "pCCW12-ERG12-tPRM9". We use pCCW12 as the promoter, tPRM9 as the terminator and the CDS of this part is ERG12. The three basic parts above make up a new transcription unit, It has the function to catalyze the conversion of mevalonate to mevalonate-P in mevalonate pathway.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 240
Illegal EcoRI site found at 1241
Illegal XbaI site found at 1323 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 240
Illegal EcoRI site found at 1241
Illegal NheI site found at 1898 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 240
Illegal EcoRI site found at 1241
Illegal BglII site found at 1260
Illegal BamHI site found at 804 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 240
Illegal EcoRI site found at 1241
Illegal XbaI site found at 1323 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 240
Illegal EcoRI site found at 1241
Illegal XbaI site found at 1323
Illegal AgeI site found at 1555 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |